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microcal itc origin analysis software  (Malvern Panalytical)


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    Structured Review

    Malvern Panalytical microcal itc origin analysis software
    Microcal Itc Origin Analysis Software, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 99/100, based on 16279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/itc+origin+analysis+software/PEAQ-ITC/pm42086723-286-4-8
    Average 99 stars, based on 16279 article reviews
    microcal itc origin analysis software - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Software:

    Article Title: α-Synuclein Sterically Stabilizes Spherical Nanoparticle-Supported Lipid Bilayers.
    Article Snippet: Isothermal Titration Calorimetry (ITC): ITC measurements were performed on a Malvern ITC200 by titrating -Synuclein into SSLB samples (with lipid concentration quantified via phosphate analysis), as previously described.29 40 μL of 100 μM (depending on sample) -Synuclein was titrated into SSLBs at nominal silica nanoparticle concentration of 1 mg/mL in one pre-injection of 0.4 μL and 19 subsequent injections of 2 μL of -Synuclein. .. Data were analyzed via the MicroCal ITC-Origin Analysis software to extract binding stoichiometry, apparent dissociation constant, and other thermodynamic parameters. ..

    Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites
    Article Snippet: .. Based on the assumption of non-interference between the two binding sites from the NMR titration experiments, we also employed a two-site independent model in the ITC analysis, referred to as the ‘Two Set of Sites’ model in MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Article Title: Deep mutational scanning of influenza A virus neuraminidase facilitates the identification of drug resistance mutations in vivo .
    Article Snippet: .. All titration data were calculated and analyzed by MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Article Title: X-ray structures of Enterobacter cloacae allose-binding protein in complexes with monosaccharides demonstrate its unique recognition mechanism for high affinity to allose.
    Article Snippet: D-Allose is an aldohexose of the C3-epimer of D-glucose, existing in very small amounts in nature, called a rare sugar.. The operon responsible for D-allose metabolism, the allose operon, was found in several bacteria, which consists of seven genes: alsR, alsB, alsA, alsC, alsE, alsK, and rpiB.. To understand the biological implication of the allose operon utilizing a rare sugar of D-allose as a carbon source, it is important to clarify whether the allose operon functions specifically for D-allose or also functions for other ligands.

    Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites
    Article Snippet: .. Each parameter was determined by analyzing row ITC data with the model, Two Set of Sites, using MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Article Title: Targeting papain-like protease for broad-spectrum coronaviruses inhibition
    Article Snippet: .. After excluding the first injection, all titration data was calculated and analyzed by MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Article Title: Biochemical Functions of the Membrane-Binding Domain of CARMIL
    Article Snippet: .. Binding constants were determined by fitting the change in enthalpy to a single site binding model using MicroCal ITC Origin analysis software. .. 100 mg 18:1 (Δ9-Cis) PC (DOPC) [1,2-dioleoyl-sn-glycero-3-phosphocholine in chloroform (Avanti Research 850375C Alabaster, AL)] and 100 mg 18:1 PS (DOPS) [1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) in chloroform (Avanti Research 840035C)] were combined and dried with Argon gas followed by further drying under vacuum.

    Article Title: Martynoside rescues 5-fluorouracil-impaired ribosome biogenesis by stabilizing RPL27A.
    Article Snippet: .. The first injection was discarded, and all subsequent obtained titration data were analyzed using MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Binding Assay:

    Article Title: α-Synuclein Sterically Stabilizes Spherical Nanoparticle-Supported Lipid Bilayers.
    Article Snippet: Isothermal Titration Calorimetry (ITC): ITC measurements were performed on a Malvern ITC200 by titrating -Synuclein into SSLB samples (with lipid concentration quantified via phosphate analysis), as previously described.29 40 μL of 100 μM (depending on sample) -Synuclein was titrated into SSLBs at nominal silica nanoparticle concentration of 1 mg/mL in one pre-injection of 0.4 μL and 19 subsequent injections of 2 μL of -Synuclein. .. Data were analyzed via the MicroCal ITC-Origin Analysis software to extract binding stoichiometry, apparent dissociation constant, and other thermodynamic parameters. ..

    Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites
    Article Snippet: .. Based on the assumption of non-interference between the two binding sites from the NMR titration experiments, we also employed a two-site independent model in the ITC analysis, referred to as the ‘Two Set of Sites’ model in MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Article Title: Biochemical Functions of the Membrane-Binding Domain of CARMIL
    Article Snippet: .. Binding constants were determined by fitting the change in enthalpy to a single site binding model using MicroCal ITC Origin analysis software. .. 100 mg 18:1 (Δ9-Cis) PC (DOPC) [1,2-dioleoyl-sn-glycero-3-phosphocholine in chloroform (Avanti Research 850375C Alabaster, AL)] and 100 mg 18:1 PS (DOPS) [1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) in chloroform (Avanti Research 840035C)] were combined and dried with Argon gas followed by further drying under vacuum.

    Nuclear Magnetic Resonance:

    Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites
    Article Snippet: .. Based on the assumption of non-interference between the two binding sites from the NMR titration experiments, we also employed a two-site independent model in the ITC analysis, referred to as the ‘Two Set of Sites’ model in MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Titration:

    Article Title: Different molecular recognition by three domains of the full-length GRB2 to SOS1 proline-rich motifs and EGFR phosphorylated sites
    Article Snippet: .. Based on the assumption of non-interference between the two binding sites from the NMR titration experiments, we also employed a two-site independent model in the ITC analysis, referred to as the ‘Two Set of Sites’ model in MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Article Title: Deep mutational scanning of influenza A virus neuraminidase facilitates the identification of drug resistance mutations in vivo .
    Article Snippet: .. All titration data were calculated and analyzed by MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Article Title: Targeting papain-like protease for broad-spectrum coronaviruses inhibition
    Article Snippet: .. After excluding the first injection, all titration data was calculated and analyzed by MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Article Title: Martynoside rescues 5-fluorouracil-impaired ribosome biogenesis by stabilizing RPL27A.
    Article Snippet: .. The first injection was discarded, and all subsequent obtained titration data were analyzed using MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Injection:

    Article Title: Targeting papain-like protease for broad-spectrum coronaviruses inhibition
    Article Snippet: .. After excluding the first injection, all titration data was calculated and analyzed by MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..

    Article Title: Martynoside rescues 5-fluorouracil-impaired ribosome biogenesis by stabilizing RPL27A.
    Article Snippet: .. The first injection was discarded, and all subsequent obtained titration data were analyzed using MicroCal ITC-ORIGIN Analysis Software (Malvern Panalytical). ..



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    <t>ITC</t> traces of CP titrated with CBR126 wt (panel A) or CBR126 mut (panel B). Upper panels are raw traces of thermograms, with differential power for each injection of CBR. Lower panels are binding isotherms, with integrated enthalpy vs molar ratio. Fitted values for K D , with a stoichiometry of one, are listed.
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    <t>ITC</t> traces of CP titrated with CBR126 wt (panel A) or CBR126 mut (panel B). Upper panels are raw traces of thermograms, with differential power for each injection of CBR. Lower panels are binding isotherms, with integrated enthalpy vs molar ratio. Fitted values for K D , with a stoichiometry of one, are listed.
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    H3Q5ser stabilizes Taf3-PHD/H3K4me2 and Taf3-PHD/H3K4me3 complexes. ( A ) Experimental (blue) and fitted (red) spectra of free, ∼50% bound, and fully bound TAF3-PHD resonances E862 and C896 for H3K4me3/H3K4me3Q5ser complex. Transfer peaks indicated with *. Experimental spectrum of E862 is also shown separately for clarity. ( B ) <t>ITC</t> analysis of GST-tagged Taf3-PHD binding to H3K4me3 in yellow and H3K4me3Q5ser in navy blue. Top panel displays the raw heat change per injection of H3 peptide. Bottom panel represents the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software.
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    Image Search Results


    ITC traces of CP titrated with CBR126 wt (panel A) or CBR126 mut (panel B). Upper panels are raw traces of thermograms, with differential power for each injection of CBR. Lower panels are binding isotherms, with integrated enthalpy vs molar ratio. Fitted values for K D , with a stoichiometry of one, are listed.

    Journal: bioRxiv

    Article Title: Biochemical Functions of the Membrane-Binding Domain of CARMIL

    doi: 10.64898/2026.01.05.697744

    Figure Lengend Snippet: ITC traces of CP titrated with CBR126 wt (panel A) or CBR126 mut (panel B). Upper panels are raw traces of thermograms, with differential power for each injection of CBR. Lower panels are binding isotherms, with integrated enthalpy vs molar ratio. Fitted values for K D , with a stoichiometry of one, are listed.

    Article Snippet: Binding constants were determined by fitting the change in enthalpy to a single site binding model using MicroCal ITC Origin analysis software.

    Techniques: Injection, Binding Assay

    H3Q5ser stabilizes Taf3-PHD/H3K4me2 and Taf3-PHD/H3K4me3 complexes. ( A ) Experimental (blue) and fitted (red) spectra of free, ∼50% bound, and fully bound TAF3-PHD resonances E862 and C896 for H3K4me3/H3K4me3Q5ser complex. Transfer peaks indicated with *. Experimental spectrum of E862 is also shown separately for clarity. ( B ) ITC analysis of GST-tagged Taf3-PHD binding to H3K4me3 in yellow and H3K4me3Q5ser in navy blue. Top panel displays the raw heat change per injection of H3 peptide. Bottom panel represents the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software.

    Journal: Nucleic Acids Research

    Article Title: Molecular determinants for recognition of serotonylated chromatin

    doi: 10.1093/nar/gkaf612

    Figure Lengend Snippet: H3Q5ser stabilizes Taf3-PHD/H3K4me2 and Taf3-PHD/H3K4me3 complexes. ( A ) Experimental (blue) and fitted (red) spectra of free, ∼50% bound, and fully bound TAF3-PHD resonances E862 and C896 for H3K4me3/H3K4me3Q5ser complex. Transfer peaks indicated with *. Experimental spectrum of E862 is also shown separately for clarity. ( B ) ITC analysis of GST-tagged Taf3-PHD binding to H3K4me3 in yellow and H3K4me3Q5ser in navy blue. Top panel displays the raw heat change per injection of H3 peptide. Bottom panel represents the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software.

    Article Snippet: ITC data were subsequently analysed and fitted with the one-site binding model in the MicroCal ITC Origin Analysis software 7.0 (Table , Figs and , , and ).

    Techniques: Binding Assay, Injection, Software

    Taf3-PHD P881 and W894 form key interaction surface with the serotonin group. ( A ) ITC analysis of GST-tagged Taf3-PHD mutants, BPTF-PHD , PHF2-PHD, or PHF8-PHD with the peptides H3K4me3 (in yellow) and H3K4me3Q5ser (in blue). Top panels display the raw heat change per injection of H3 peptide. Bottom panels represent the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software. ( B ) Multiple sequence alignment (MSA) of human PHD fingers for which high-resolution structures in complex with H3K4me3 are available (ASH1L PDB: 8VLF; BPTF(2) PDB: 2FUU; DIDO1 PDB: 4L7X; ING1 PDB: 2QIC; ING4 PDB: 2PNX/2VNF; ING5 PDB: 3C6W; KMT2E PDB: 4L58; PHF2 PDB: 3KQI; PHF13 PDB: 3O7A; UHRF1 PDB: 3SOW). Consensus Cys4–His–Cys3 positions are highlighted in grey and indicated on top. Locations of the secondary structure elements of TAF3-PHD are indicated on top. S880 and A902 are highlighted in purple, while P881 and W894 are highlighted in yellow.

    Journal: Nucleic Acids Research

    Article Title: Molecular determinants for recognition of serotonylated chromatin

    doi: 10.1093/nar/gkaf612

    Figure Lengend Snippet: Taf3-PHD P881 and W894 form key interaction surface with the serotonin group. ( A ) ITC analysis of GST-tagged Taf3-PHD mutants, BPTF-PHD , PHF2-PHD, or PHF8-PHD with the peptides H3K4me3 (in yellow) and H3K4me3Q5ser (in blue). Top panels display the raw heat change per injection of H3 peptide. Bottom panels represent the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software. ( B ) Multiple sequence alignment (MSA) of human PHD fingers for which high-resolution structures in complex with H3K4me3 are available (ASH1L PDB: 8VLF; BPTF(2) PDB: 2FUU; DIDO1 PDB: 4L7X; ING1 PDB: 2QIC; ING4 PDB: 2PNX/2VNF; ING5 PDB: 3C6W; KMT2E PDB: 4L58; PHF2 PDB: 3KQI; PHF13 PDB: 3O7A; UHRF1 PDB: 3SOW). Consensus Cys4–His–Cys3 positions are highlighted in grey and indicated on top. Locations of the secondary structure elements of TAF3-PHD are indicated on top. S880 and A902 are highlighted in purple, while P881 and W894 are highlighted in yellow.

    Article Snippet: ITC data were subsequently analysed and fitted with the one-site binding model in the MicroCal ITC Origin Analysis software 7.0 (Table , Figs and , , and ).

    Techniques: Injection, Binding Assay, Software, Sequencing